Diverse Escherichia coli lineages from domestic animals carrying colistin resistance gene mcr-1 in an Ecuadorian household

Diverse Escherichia coli lineages from domestic animals carrying colistin resistance gene mcr-1 in an Ecuadorian household

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Objective: The aim of this study was to detect potential animal reservoirs of Escherichia coli carrying the mcr-1 gene in an Ecuadorian household.Methods: The mobile colistin-resistance gene, mcr-1, was first detected in Ecuador in a commensal E.coli isolate from a boy.

A cross-sectional study was performed to detect the possible source of colistin-resistant E.coli in the boy’s household.Faecal swabs and soil faecal Happy samples were collected from companion animals.

Samples were plated on selective media to isolate colistin-resistant E.coli and isolates were submitted to PCR detection of mcr-1, pulsed field gel electrophoresis (PFGE), and multi-locus sequences typing (MLST).Moreover, the genomes of all the isolates were sequenced.

Results: Three different colistin-resistant E.coli sequence types (ST3941, 1630 and 2170), corresponding to three PFGE patterns, Vacuum Composite Filter were obtained from a chicken and two dogs; these isolates were different from the human isolate (ST609).By whole-genome sequencing, the mcr-1.

1 gene was found on IncI2 plasmids with very high nucleotide identity.Conclusions: Our results indicate a polyclonal dissemination of mcr-1.1 in the environment surrounding the first MCR-producing E.

coli strain reported in Ecuador.Our findings support the idea of lateral dissemination of mcr-1.1 gene between unrelated E.

coli isolates.